NEET Biotechnology & Its Applications 5 Practice Tests • 20 MCQs Each SEPTEMBER 1

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Biotechnology & Its Applications

5 Practice Tests • 20 MCQs Each • Answers revealed after submission

Test 1

20 Questions • 20 Marks

Biotechnology & Its Applications

Q1. Louis Pasteur demonstrated that:

A. Early life came from outer space. B. Non-living chemicals produced living molecules. C. Life comes from pre-existing life. D. Life originated spontaneously. 1Mark

Q2. Who among the following challenged the patent right granted to the University of Mississippi Medical Centre for 'use of turmeric in wound healing'?

A. Mr. Ajay Phadke B. Ms. Vandana Shiva C. Dr. Venugopalan D. Dr. R.A. Mashelkar 1Mark

Q3. ELISA technique is based on the principle of: 1. DNA replication. 2. Antigen and Antibody interaction. 3. Pathogen and Antigen interaction. 4. Antigen and Protein interaction.

A. DNA replication. B. Antigen and Antibody interaction. C. Pathogen and Antigen interaction. D. Antigen and Protein interaction. 1Mark

Q4. The first gene-sequenced crop is:

A. Wheat B. Tobacco C. Rice D. Cotton 1Mark

Q5. After the completion of biosynthetic stage in a bioreactor, the product undergoes a series of processes before it is ready for marketing. List of the processes is given below. Identify the option that gives the correct sequence of the processes carried out: 1. Purification of product 2. Formulation with suitable preservative 3. Separation of product 4. Clinical trial of product

A. (ii) → (iii) → (i) → (iv) B. (iii) → (ii) → (i) → (iv) C. (iii) → (i) → (ii) → (iv) D. (i) → (iii) → (iv) → (i) 1Mark

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Q6. Which one of the following is used during ‘RNA i’ process, to silence the desired gene?

A. dsDNA B. dsRNA C. rDNA D. DNA polymerase 1Mark

Q7. Which one of the following products was produced as a result of DNA manipulation in the first transgenic cow ‘Rosie’?

A. α - 1 - antitrypsin B. α - lactalbumin C. β - lactglucose D. α - deaminase 1Mark

Q8. 'Cry’ 'protein’ coded by gene Cry IAb controls:

A. Cotton bollworm. B. Corn borer. C. Tobacco budworm. D. Mosquito. 1Mark

Q9. Micropropagation can be achieved by: 1. Self-pollination. 2. Asexual reproduction. 3. Tissue culture. 4. Vegetative propagation.

A. Self-pollination. B. Asexual reproduction. C. Tissue culture. D. Vegetative propagation. 1Mark

Q10. Silencing of a gene could be achieved through the use of:

A. Short interfering RNA (RNAi). B. Antisense RNA. C. By both. D. None of the above. 1Mark

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Q11. Banting and Best successfully purified insulin from the pancreas of:

A. Guinea pig B. Dog C. Cow D. Buffalo 1Mark

Q12. Today, transgenic models exist for many human diseases which includes. 1. Cancer. 2. Cystic fibrosis. 3. Rheumatoid arthritis. 4. Alzhiemer’s disease.

A. (i) and (iii) only. B. (ii) and (iii) only. C. (i), (ii) and (iii) only. D. All of these. 1Mark

Q13. The transgenic animals are those which have:

A. Foreign DNA in some cells. B. Foreign DNA in all of their cells. C. Foreign RNA in all of their cells. D. Both (a) and (c). 1Mark

Q14. The source of alkaline phosphatase enzyme:

A. BAP. B. CAP. C. YAC. D. Both (a) and (b). 1Mark

Q15. SCID is caused by defective gene coding for enzyme ______________:

A. Adenosine deaminase B. Adenosine transaminase C. Adenosine transferase D. Guanosine transaminaseGuanosine deaminase 1Mark

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Q16. Transgenic plants receive genes from which of the following organisms?

A. Insects B. Bacteria C. Virus D. All of the above 1Mark

Q17. Globular protein of ~ 6kDaconsisting of 51 amino acids arranged in two polypeptide chains held by disulphide bridge is:

A. Insulin B. Fibrinogen C. Glucagon D. Keratin 1Mark

Q18. E. coli are used in production of:

A. Rifampicin B. LH C. Ecdysone D. Interferon 1Mark

Q19. Product of biotechnology is:

A. Transgenic crops (GM crops) B. Humulin C. Biofertilizer D. All of the above 1Mark

Q20. Restriction enzymes are also called:

A. Vectors. B. Carriers. C. Molecular scissors. D. Molecular makers. 1Mark

Test 2

20 Questions • 20 Marks

Biotechnology & Its Applications

Q1. The trigger for activation of toxin of Bacillus thuringiensis is:

A. Alkaline pH of gut. B. Mechanical action in the insect gut. C. High temperature. D. Acidic pH of stomach. 1Mark

Q2. Production of human protein in bacteria by genetic engineering is possible because:

A. Mechanism of gene regulation is identical in humn and bacteria. B. Bacterial cell can undertake RNA splicing. C. Genetic code is universal. D. None of these. 1Mark

Q3. Bt cotton is not:

A. Resistant to all pesticides. B. A bacterial gene expressing system. C. Insect resistant. D. A GM plant. 1Mark

Q4. What is searched from the patient's blood in ELISA method for the detection of AIDS?

A. Antigen B. Antibody C. No. of WBC D. No. of RBC 1Mark

Q5. Production of a human protein in bacteria by genetic engineering is possible because:

A. Bacterial cell can carry out the RNA splicing reactions. B. The human chromosome can replicate in bacterial cell. C. The mechanism of gene regulation is identical in humans and bacteria. D. The genetic code is universal. 1Mark

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Q6. GEAC stands for:

A. Genome Engineering Action Committee. B. Ground Environment Action Committee. C. Genetic Engineering Approval Committee. D. Genetic and Environment Approval committee. 1Mark

Q7. Tobacco plants resistant to a nematode have been developed by the introduction of DNA that produced (in the host cells).

A. Both sense and anti-sense RNA. B. A particular hormone. C. An antifeedant. D. A toxic protein. 1Mark

Q8. The polymerase chain reaction (PCR) is a powerful technique to:

A. Mutate genes B. Multiply genes C. Inhibit DNA synthesis D. Induce protein synthesis 1Mark

Q9. What is true of Bt toxin?

A. The inactive protoxin get converted into active form in the insect gut. B. Bt protein exists as active toxin in bacillus. C. The concerned bacillus has antitoxins. D. None of these. 1Mark

Q10. Gene therapy has been successful in curing genetic diseases in laboratory animals through

A. Exposure to X-ray to rectify the defective gene B. Replacing the defective gene with a functional gene C. Oral delivery of genes D. Use of therapeutic medicines to rectify the defective genes 1Mark

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Q11. Expand ELISA:

A. Enzyme linked inductive assay B. Enzyme linked ion sorbent assay C. Enzyme linked immunosorbent assay D. None of the above 1Mark

Q12. The source of DNA polymerase used in PCR: 1Mark

A. Bacteria. B. Bacteriophage. C. Yeast. D. Fungus

Q13. Example/s of biopiracy of Indian plants is/ are _________:

A. Basmati rice B. Turmeric C. Neem D. All of the above 1Mark

Q14. The process of RNA interference has been used in the development of plants resistant to?

A. Insects B. Nematodes C. Fungi D. Viruses 1Mark

Q15. Which bacterium is used in the production of insulin by genetic engineering?

A. Escherichia B. Mycobacterium C. Rhizobium D. Saccharomyces 1Mark

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Q16. In some children, ADA deficiency can be cured by:

A. Bone marrow transplantation. B. Enzyme replacement therapy. C. Both (a) and (b). D. None of the above. 1Mark

Q17. Transgenic animals are extensively used for all the following procedures except:

A. Bioremediation B. Chemical safety C. Vaccine safety D. Toxicity test 1Mark

Q18. The resistant variety of rice to bacterial blight is:

A. Jaya B. TMK-6 C. IR-20 D. IR-8 1Mark

Q19. Genetic engineering has been successfully used for producing:

A. Animals with inferior traits. B. Transgenic mice for testing safety of polio vaccine before use in humans C. Animals that have the same genetic constitution. D. None of the above 1Mark

Q20. Biopiracy is widespread in:

A. Asia B. Africa C. America D. All of the above 1Mark

Test 3

20 Questions • 20 Marks

Biotechnology & Its Applications

Q1. Producing a giant mouse in the laboratory was possible through.

A. Gene mutation. B. Gene manipulation. C. Gene synthesis. D. Gene duplication. 1Mark

Q2. Transgenic plants are:

A. Produced by a somatic embryo in an artificial medium. B. Generated by introducing foreign DNA into a cell and regenerating a plant form. C. Produced after protoplast fusion in an artificial medium. D. Grown in an artificial medium after hybridization in the field. 1Mark

Q3. An abnormal gene is replaced by normal gene. It is called __________:

A. Gene therapy B. Cloning C. Mutation D. None of the above 1Mark

Q4. Meristem culture is practised on horticulture to get:

A. Somaclonal variation B. Haploids C. Virus-free plants D. Slow-growing callus 1Mark

Q5.

A. Can be ss DNA but not ss RNA. B. Either RNA or DNA. 1Mark C. A single stranded DNA. D. A single stranded RNA.

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NEET English Medium

Q6. PCR technique was invented by:

A. Boyer. B. Sanger. C. Karry Mullis. D. None of these. 1Mark

Q7. Pathophysiology is the:

A. Study of normal physiology of host. B. Study of physiology of pathogen. C. Study of altered physiology of host. D. None of the above. 1Mark

Q8. Which of the following principle is associated with the ethics of animal use in genetic engineering?

A. Reduction of animal use B. Refinement of husbandry C. Replacement of animal with non-animal alternatives D. All of the above 1Mark

Q9. Restriction endonuclease is employed for cutting.

A. Double strand DNA. B. RNA fragment. C. mRNA. D. A single strand DNA. 1Mark

Q10. Maximum number of existing transgenic animals is of:

A. Pig B. Fish C. Mice D. Cow 1Mark

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Q11. What is true about Bt toxin?

A. The activated toxin enters the ovaries of the pest to sterilise it and thus prevent its multiplication. B. The concerned Bacillus has antitoxins. C. The inactive protoxin gets converted into active form in the insect gut. D. Bt protein exists as active toxin in the Bacillus.` 1Mark

Q12. Bt toxin kill the larvae of certain insects:

A. By binding of activated toxin on mid gut epithelial cells, creating pores, leading to swelling and lysis. B. By stopping transcription of larval cells. C. By altering central dogma taking place in the cells of gut of larva. D. By stopping protein synthesis. 1Mark

Q13. Which of the following is the ethics for the research with animals?

A. and B. 1Mark C. Any animal can be used D. Both

Q14. Animal suffering during testing can be minimized by which of these principles of ethics of animal biotechnology?

A. Reduction B. Refinement C. Replacement D. All of the above 1Mark

Q15. Genetically engineered bacteria are being employed for production of:

A. Thyroxine B. Human insulin C. Cortisol D. Epinephrine 1Mark

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Q16. Choose the correct option regarding Retrovirus.

A. A DNA virus that can synthesise RNA during infection. B. An RNA virus that can synthesise DNA during infection. C. A ssDNA virus. D. A dsRNA virus. 1Mark

Q17. Which of the following biomolecules can be analysed by ELISA test?

A. Peptides B. Hormones C. Antibodies D. All of the above 1Mark

Q18. Which of the following is the key principle of bioethics?

A. Animal rights B. Environmental ethics C. Autonomy D. All of the above 1Mark

Q19. The competitive indirect ELISA differs from direct ELISA, as in competitive indirect ELISA:

A. Unlabeled antibody is incubated in the presence of its antigen. B. Labelled antibody is incubated in the presence of its antigen. C. Labelled antigen is incubated in the presence of its antibody. D. None of the above. 1Mark

Q20. Some multinational companies patent biological sample of other country without permission is called as:

A. Bioweapon B. Bioethics C. Biopiracy D. Biopatent 1Mark

Test 4

20 Questions • 20 Marks

Biotechnology & Its Applications

Q1. Who initiates the campaign against basmati rice?

A. and B. 1Mark C. Navdanya D. Both

Q2. Biopiracy of Indian plant by USA is:

A. Mustard B. Melon fruit seeds C. Jasmine rice D. All of the above 1Mark

Q3. The T, plasmid used for producing transgenic plants is found in:

A. Azotobacter. B. Rhizobium. C. Azospirillum. D. Agrobacterium. 1Mark

Q4. More than 95% of transgenic animals are:

A. Rabbits B. Mice C. Fish D. Cows 1Mark

Q5. In genetics engineering which is used for transfer of genes from one cell to another.

A. Virus B. Probe C. Plasmid D. Vector 1Mark

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Q6. Crystals of Bt toxin produced by some bacteria do not kill the bacteria themselves because:

A. Bacteria are resistant to the toxin. B. Toxin is immature. C. Toxin is inactive. D. Bacteria encloses toxin in a special sac. 1Mark

Q7. Genetic material with potential value is:

A. DNA B. Genetic resources C. RNA D. None of these 1Mark

Q8. Gene therapy for the first time was clinically done for:

A. ADA deficiency. B. Rheumatoid fever. C. Diabetes. D. Alzheimer's disease. 1Mark

Q9. The first clinical gene therapy was done for the treatment of:

A. AIDS. B. Cancer. C. Cystic fibrosis. D. SCID (Servere Combined Immuno Deficiency resulting form deficiency of ADA) 1Mark

Q10. The purpose of biopiracy is:

A. Introduction of new plant varieties B. Introduction of new living organisms C. Production of pharmaceuticals D. All of the above 1Mark

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JEE Practice

Q11. The bacterium Bacillus thuringiensis is widely used in contemporary biology as:

A. Agent for production of dairy products. B. Source of industrial enzyme. C. Indicator of water pollution. D. Insecticide. 1Mark

Q12. PCR and RFLP are employed in ________:

A. DNA sequencing B. Genetic engineering C. Study of enzymes D. Genetic transformation 1Mark

Q13. Insulin has 51 amino acids arranged in _____:

A. Single polypeptide B. Two polypeptides of 21 and 30 amino acids C. Two polypeptides of 25 and 26 amino acids D. Three polypeptides having 15, 16 and 20 amino acids 1Mark

Q14. The first transgenic cow:

A. Rosie. B. Anandi. C. Rama. D. Nandi. 1Mark

Q15. What is the function of the polymerase chain reaction in genetic engineering?

A. Cut DNA into many fragments B. Carry DNA into a new cell 1Mark C. Link together newly joined fragments of DNA D. Make millions of copies of a specific segment of DNA

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NEET Previous Year Questions

Q16. High levels of aspartic acid, low nitrogen and sugar content in maize plants prevent the attack by:

A. Aphids B. Jassids C. Boll worms D. Stem borers 1Mark

Q17. Which of the following enzyme is used in ELISA?

A. and B. 1Mark C. Hexokinase D. Both

Q18. Cry-I endotoxins obtained from Bacillus thuringiensis are effective against.

A. Mosquitoes B. Flies C. Nematodes D. Bollworms 1Mark

Q19. antitrypsin is:

A. Used to treat arthritis. B. Used to treat emphysema. C. An enzyme. D. An antacid. 1Mark

Q20. The site of production of ADA in the body is:

A. Bone marrow. B. Blood plasma. C. Monocytes. D. Lymphocytes. 1Mark

Test 5

20 Questions • 20 Marks

Biotechnology & Its Applications

Q1. Inhibitory antigen is present in which of the following technique?

A. Direct ELISA B. Indirect ELISA C. Competitive ELISA D. All of the above 1Mark

Q2. Before animal testing _________should be given to the animals: 1Mark α−1

A. Anesthesia B. Analgesics C. Antibiotics D. Antiseptics

Q3. Important objective of biotechnology in agriculture section is:

A. To produce pest resistant varieties of plant B. To increase the nitrogen content C. To decrease the seed number D. To increase the plant weight 1Mark

Q4. An improved variety of transgenic basmati rice:

A. 1Mark B. Give high yield but has no characteristic aroma C. Does not require chemical fertilizers and growth hormones D. Give high yield and is rich in vitamin

Q5. In Bt cotton, the Bt toxin present in plant tis- sue as pro-toxin is converted into active toxin due to:

A. Acidic pH of the insect gut. B. Action of gut micro-organisms. C. Presence of conversion factors in insect gut. D. Alkaline pH of the insect gut. 1Mark

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Q6. The crops engineered for glyphosate are re- sistant/ tolerant to:

A. Bacteria B. Insects C. Herbicides D. Fungi 1Mark

Q7. Which of the following is a term used for the specific substance whose presence is being quantitatively or qualitative analysed? 1Mark

A. Substrate B. Analyte C. Antibody D. None of the above

Q8. Which body of the Government of India regu- lates GM research and safety of introducing GM organisms for public services?

A. Indian Council of Agricultural Research. B. Genetic Engineering Approval Committee. C. Research Committeeon Genetic Manipulation. D. Bio-safety committee. 1Mark

Q9. RNA interference (RNAi) technique has been devised to protect the plants from nematode is silenced by ______ produced by the host plant.

A. dsDNA. B. ssDNA. C. dsRNA. D. Target proteins. 1Mark

Q10. Maturation of proinsulin into insulin takes place after:

A. Joining of c-peptide. B. Addition of disulphide bridge. C. Joining of c-peptide. D. Maturation of proinsulin into insulin takes place after. 1Mark

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Q11. All of the followings are the ethical issues with the animal testing except:

A. Pain B. Disease C. Infertility D. All of the above 1Mark

Q12. The first human hormone produced by re- combinant DNA technology is:

A. Insulin B. Estrogen C. Thyroxine D. Progesterone 1Mark

Q13. In RNAi, genes are silenced using:

A. ss DNA. B. ds DNA. C. ds RNA. D. ss RNA. 1Mark

Q14. Abnormal gene is replaced by normal genes through:

A. Gene therapy B. Medicines C. Cloning D. Radiation 1Mark

Q15. How does proinsulin become mature?

A. By adding a short peptide. B. By removal of C-peptide. C. By cutting into 2 halves. D. By changing its 3D shape. 1Mark

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Q16. Which of the following phenomena can be related to ELISA?

A. Agglutination B. Clotting C. Neutralization D. None of the above 1Mark

Q17. DNA or RNA segment tagged with a radioactive molecule is called _________.

A. Vector B. Probe C. Clone D. Plasmid 1Mark

Q18. In an experiment the gene of bioluminescence from firefly has been successfully transferred into a plant as a result of which it started to glow. In the terminology of the molecular genetics, the plant is called ___________:

A. Firefly plant B. Hybrid plant C. Transgenic plant D. Bioluminescent plant 1Mark

Q19. The first clinical gene therapy was given in 1990 to a 4 years old girl with enzyme deficiency of:

A. Adenosine deaminase (ADA). B. Tyrosine oxidase. C. Monamine oxidase. D. Glutamate dehydrogenase. 1Mark

Q20. The method of biopiracy is _________:

A. Illegal collection of biological sample B. Legal collection of biological sample C. Biopatent D. None of the above 1Mark

Questions are based on the uploaded “Biotechnology and its Applications MCQ QA” material.

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Access Tamil learning and preparation resources for Classes 10, 11 and 12.

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ONLINE QUIZ

Online Quiz

Practice interactive online quizzes and test your knowledge with MCQ questions.

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